Structural Analysis of Polysaccharides in Chinese Lacquer by NMR Spectroscopy.

Solution NMR Spectroscopy of Food Polysaccharides

Many polysaccharides are allowed for direct food use, where they serve a number of useful functions including dietary fiber, bulking agent, thickener, encapsulant, gelling agent, foam and emulsion stabilizer, protective colloid, emulsifier and suspending agent, adhesive and binder, flocculant, swelling agent, film/coat former, or syneresis inhibitor. Many of these polysaccharides have complex s...

The structural analysis of protein-protein interactions by NMR spectroscopy.

A comprehensive understanding of protein-protein interactions is an important next step in our quest to understand how the information contained in a genome is put into action. Although a number of experimental techniques can report on the existence of a protein- protein interaction, very few can provide detailed structural information. NMR spectroscopy is one of these, and in recent years seve...

Structural Studies of Some Bacterial Lipopolysaccharides and Extracellular Polysaccharides using NMR Spectroscopy and Mass Spectrometry

Structural studies of proteins by paramagnetic solid-state NMR spectroscopy.

Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron-nucleus distances on the ∼20 Å length scale, far beyond that normally probed through measurements of nuclear dipolar couplings. Such data are especially vital in the context of structural studies of proteins and other biological molecules th...

Structural characterization of polyglutamine fibrils by solid-state NMR spectroscopy.

Protein aggregation via polyglutamine stretches occurs in a number of severe neurodegenerative diseases such as Huntington's disease. We have investigated fibrillar aggregates of polyglutamine peptides below, at, and above the toxicity limit of around 37 glutamine residues using solid-state NMR and electron microscopy. Experimental data are consistent with a dry fibril core of at least 70-80 Å ...